![]() ![]() G C A N N N N N T G C C G T N N N N N A C G ![]() Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different PfoI sites may not be compatible. Sticky ends from different XcmI sites may not be compatible.īsrGI is typically used at 37☌, but is even more active at 60☌.ĪpoI is typically used at 50☌, but is 50% active at 37☌.īsiWI is typically used at 55☌, but is 50% active at 37☌.
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